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Abstract #8551 Published in IGR 5-1
Evaluation of adverse ocular effects of 5-fluorouracil by using human corneal epithelial cell cultures
Huhtala A; Tahti H; Salminen L; Uusitalo HJournal of Toxicology – Cutaneous and Ocular Toxiclogy 2002; 21: 283-292
5-Fluorouracil (5-FU) is commonly used in ophthalmology for suppressing fibroblast activity after glaucoma surgery. Adverse effects on corneal epithelial cells have been reported to relate to 5-FU therapy. The effects of 5-FU were evaluated in vitro on SV40-immortalized human corneal epithelial cell (HCE) cultures with two cytotoxicity tests: WST-1 assay as an index of cell proliferation, and lactate dehydrogenase (LDH) assay as an index of plasma membrane integrity. The cells were exposed to 5-FU with various concetrations in serum-free medium and in medium containing 15% (v/v) fetal bovine serum (FBS) for one, 24, 48 and 72 hours. One-hour exposure had no effect on HCE cells. Longer exposures caused dose-dependent inhibition of cell proliferation. Exposure to 5 mg/ml 5-FU lowered the cell number to 50% of controls after 24 hours of treatment, and resulted to complete cell death after 72 hours. Serum protected the cells for 24 hours, but after longer exposure times, the protective nature of serum disappeared. 5-FU only had minor effects on LDH release. LDH leakage was at its peak after 48 hours of treatment.
Dr. A. Huhtala, University of Tampere, Medical School, FIN-33014 Tampere, Finland. Ilanhu@uta.fi
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
