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Abstract #8604 Published in IGR 5-1
Bovine trabecular meshwork cells express insulin-like growth factor-1 (IGF-1) mRNA and protein
Cao Y; Phaffl MW; Da B; Wei HOphthalmologe 2002; 99: 555-558
BACKGROUND: The purpose of this study was to determine whether cultured bovine trabecular meshwork cells and trabecular tissue ex vivo express insulin-like growth factor-1 (IGF-1) mRNA and protein. METHODS: The reverse transcriptase-polymerase chain reaction (RT-PCR) was used for detection of IGF-1 mRNA.To detect the protein on the cells an IGF-1-specific immunohistochemical stain was used on trabecular meshwork cells. RESULTS: A single 240 bp RT-PCR product was obtained, the RT-PCR product was verified by sequencing, and the derived sequence was homologous to the known bovine sequence. IGF-1 immunostaining was positive in the cytoplasm of trabecular meshwork cells. CONCLUSIONS: The authors conclude that trabecular meshwork cells produce IGF-1 mRNA and contribute to the presence of IGF-1 protein in the trabecular meshwork microenvironment as well as aqueous humor. Trabecular meshwork cells were affected by IGF-1 not only through paracrine but also through autocrine action. Whether regulations in IGF-1 production may contribute to the pathogenesis of primary open-angle glaucoma and the possibility of promoting the autocrine action of IGF-1 by trabecular meshwork cells to treat the disease is worth further investigation.LA: German
Dr. Y. Cao, Department of Ophthalmology, Union Hospital, Huazhong Univ. of Sci./Technology, Wuhan, China. ytsao@public.wh.hbcn
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
3 Laboratory methods
