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Abstract #8610 Published in IGR 5-1
The inhibition effect of photodynamic on human Tenon capsule fibroblast cells
Chen H; Ge J; Guo Y; Jin C; Lan Y; Lin MChinese Journal of Ophthalmology 2003; 39: 160-
OBJECTIVE: To investigate the inhibition of the photodynamic effect on human tenon capsule fibroblast cells in vitro. METHODS: Human tenon capsule fibroblast cells were divided into nine groups, Groups A to I, each with four wells. Groups A to G: each group received photosensitizer benzoporphyrin derivative monoacid ring A (BPD) with end concentrations of 2.50 x 103, 1.25 x 103, 0.62 x 103, 0.31 x 103, 0.16 x 103, 0.08 x 103, 0.04 x 103 g/L, respectively. Then the culture cells were irradiated by a 689-nm diode laser with a dosage of 2.4 J/cm2 after BPD for 15 minutes. Group H was trated with mitomycin C (MMC) at a concentration of 0.2 g/L. Group I was used as a control and received no treatment. All the cells were kept cultured for a further 24 hours and then MTT colorometric assay was used to measure the relative inhibitory rate of the photodynamic effect on the cells. RESULTS: There was a statistical significance between Groups A to H and Group I using the method of one-way analysis of variance. When compared to Group I, the relative inhibitory rates of Groups A to G were 93.3, 91.0, 90.3, 87.1, 66.0, 41.6, and 12.5%, respectively, and the inhibitory rate of Group H (MMC) was 93.0%. CONCLUSIONS: The photodynamic effect can inhibit the proliferation of human tenon capsule fibroblast cells in vitro. The inhibitory rate appears to be dependent on the concentration of the photosensitizer.LA: Chinese
Dr. H. Chen, Zhongshan Ophthalmic Center, Zhongshan University, Guangzhou 510060, China. hy-c@163.net
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
