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Abstract #86647 Published in IGR 21-2
Higher TGF-β1, TGF-β2, MMP-2 and TIMP-1 Levels in the Aqueous Humor of the Patients with Acute Primary Angle Closure
Chen Y; Yan H; Li G; Zhang YOphthalmic Research 2020; 0:
PURPOSE: To assess the quantitative differences in the levels of members of the transforming growth factors beta (TGF-β), matrix metalloproteinase (MMP), and tissue inhibitor of MMP (TIMP) families in the aqueous humor (AH) of patients who experienced acute primary angle closure (APAC) compared with levels in patients with cataract only. METHODS: AH samples were collected from 26 patients with APAC and cataract as well as 26 patients with age-related cataract only. Multiplex assays were used to measure the concentrations of TGF-β1, -2, and -3; MMP-1, 2, -7, -9, and -10; and TIMP-1 and -2. RESULTS: The concentrations of TGF-β1, TGF-β2, MMP-2, (p<0.001) and TIMP1 were significantly higher (all P<0.001) in AH samples from patients with APAC versus cataract only. Conversely, the AH concentrations of MMP-7 (P=0.524), MMP-9 (P=0.103), MMP-10 (P=0.111), and TIMP-2 (P=0.059) did not differ significantly between the groups. The concentrations of TGF-β3 and MMP-1 were below the respective detection limits in most AH samples. CONCLUSION: The AH levels of TGF-β1, TGF-β2, MMP-2, and TIMP-1 were elevated in eyes with APAC. Such altered protein levels could induce abnormal deposition of extracellular matrix in the trabecular meshwork, resulting in an increase in aqueous outflow resistance, and thereby, providing a possible explanation of the mechanism of residual glaucoma after cataract surgery.
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Classification:
9.3.1 Acute primary angle closure glaucoma (pupillary block) (Part of: 9 Clinical forms of glaucomas > 9.3 Primary angle closure glaucomas)
2.6.3 Compostion (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics)
3.7 Biochemistry (Part of: 3 Laboratory methods)
3.9 Pathophysiology (Part of: 3 Laboratory methods)