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Abstract #8689 Published in IGR 5-1
Optineurin gene expression level in human trabecular meshwork does not change in response to pressure elevation
Kamphuis W; Schneemann AOphthalmic Research 2003; 35: 93-96
Mutations in the gene optineurin (OPTN) have been associated with primary-open angle glaucoma. The authors present a study on the level of OPTN gene expression in the human trabecular meshwork in response to increased perfusion pressure in the anterior chamber perfusion model of the human eye. Perfusion pressure was raised from 10-30 mmHg for periods ranging between one and 24 hours. OPTN transcript levels in the trabecular meshwork were determined using real-time quantitative polymerase chain reaction. The results show no statistically significant alteration of the OPTN transcript level after raising the pressure. Moreover, no changes were detected in the transcript levels of the three known OPTN isoforms. This result shows that enhanced pressure levels do not lead to rapid changes in gene expression levels of OPTN in human trabecular meshwork. This suggests that alterations in OPTN gene expression are not involved in the mechanisms regulating aqueous humor outflow after an increase in intraocular eye pressure.
Dr. W. Kamphuis, Netherlands Ophthalmic Research Institute, Meibergdreef 47, NL-1105 BA Amsterdam, The Netherlands. w.kamphuis@ioi.knaw.nl
Classification:
1.2 Population genetics (Part of: 1 General aspects)
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
