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Abstract #9133 Published in IGR 5-2
The effects of theophylline on the adherence and cytoskeleton of bovine trabecular meshwork cells in culture
Wang L; Zhang D; Zhu XChinese Ophthalmic Research 2003; 21: 279-281
OBJECTIVE: To observe the effects of theophylline on cell proliferation, cytoskeleton and the expression of catenin of cultured bovine trabecular cells, and to understand further the mechanism of theophylline in reducing intraocular pressure (IOP). METHODS: Bovine trabecular meshwork cells were primarily cultured and subcultured. Various doses (0, 1, 10, 50 mmol/L) of theophylline were separately added to the third passage cells for four and eight hours. Actin and tubulin were immunohistochemically stained by SABC methods. Cell proliferation was tested by the MTT method. The amount of catenin expression was semi quantitatively evaluated by ELISA. RESULTS: Theophylline inhibited the proliferation of cultured bovine trabecular meshwork cells. The morphology of cells changed from flat to spindle like, cells body rounded up. The arrangement of actin and tubulin changed. There were specks of staining. The expression of catenin was inhibited by theophylline. CONCLUSIONS: Theophylline can inhibit cell proliferation, influent the cytoskeleton, cause loss of inter-cell adherence, and thereby create low IOP. LA: Chinese
Dr. L. Wang, Ophthalmology Institute of Xi'an, Xi'an 710002, China. w17283@sina.com.en
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)
