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Abstract #9134 Published in IGR 5-2
The effects of verapamil on the proliferation and phagocytosis of bovine trabecular meshwork cells
Chen F; Wei H; Zeng YChinese Ophthalmic Research 2003; 21: 250-253
OBJECTIVE: To study the effects of verapamil on the proliferation and phagocytosis of bovine trabecular meshwork cells (BTMC). METHODS: The effects of verapamil on the proliferation of BTMC were assessed by MTT and 3H TDR at 0. 0001, 0. 0005, 0. 001, 0. 005, 0. 01, 0. 05 and 0. 1 mg/ml. The effects of verapamil equal to or less than 0. 01 mg/ml on phagocytosis of BTMC was quantitatively measured by using latex beads as markers. RESULTS: Verapamil less than 0. 01 mg/ml had no significant effects on the proliferation of BTMC (p > 0. 05). Equal to or higher than 0. 01 mg/ml of verapamil inhibited the proliferation of BTMC in a dose-dependent manner (p < 0. 05). IC was 0. 032 3 mg/ml (MTT) and 0. 025 9 mg/ml (3H TDR), respectively. 0. 001, 0. 005, 0. 01, and 0.01 mg/ml of verapamil promoted the phagocytosis of BTMC in a dose-dependent manner (p < 0. 05). CONCLUSIONS: A concetration of (1607 ± 272) ng/ml in aqueous humor is obtained by using the eye drop of 0.125% verapamil. However this concentration of verapamil does not inhibit the proliferation of BTMC, the concentration equal to or less than 0. 01 mg/ml should be selected in following experiments. In addition, verapamil may be expected to treat primary open-angle glaucoma by blocking the pathogenesis by promoting the phagocytosis of BTMC and subsequently decreasing resistance to the outflow of aqueous humor. LA: Chinese
Dr. F. Chen, Department of Ophthalmology, Union Hospital, Huazhong Technique University, Wuhan 430022, China. chenfei1972@hotmail.com
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)