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Abstract #94457 Published in IGR 22-2
Agonistic 2-Adrenergic Receptor Autoantibodies Characterize the Aqueous Humor of Patients With Primary and Secondary Open-Angle Glaucoma
Hohberger B; Wörn M; Lämmer R; Mahajan A; Mardin C; Schötzer-Schrehardt U; Kunze R; Herrmann M; Wallukat GFrontiers in immunology 2021; 12: 550236
PURPOSE: Agonistic 2-adrenergic receptor autoantibodies (2-agAAbs) were recently observed in sera of patients with ocular hypertension (OHT), primary (POAG), and secondary open-angle glaucoma (SOAG), yet not in healthy controls (HCs). It was the aim of the present study to investigate the presence of 2-agAAb in aqueous humor (AH) samples of OAG patients and to correlate these with the corresponding 2-agAAb serum data. MATERIAL AND METHODS: Thirty-nine patients (21 male, 18 female) were recruited from the Department of Ophthalmology, University of Erlangen-Nürnberg: twenty-one POAG, 18 SOAG. Aqueous humor samples were collected during minimal invasive glaucoma surgery. Serum and AH samples were analyzed for 2-agAAb by a bioassay quantifying the beating rate of cultured cardiomyocyte (cut-off: 2 U). RESULTS: Thirty-six of 39 (92.3%) and 34 of 39 (87.2%) of OAG patients showed a 2-agAAb in their sera and AH samples, respectively. All 2-agAAb AH-positive OAG patients were also seropositive. We also observed a 2-agAAb seropositivity in 95 and 89% of patients with POAG and SOAG, respectively. Beta2-agAAbs were seen in 86% (POAG) and 78% (SOAG) of AH samples. The 2-agAAb adrenergic activity was increased in the AH of patients with POAG (6.5 ± 1.5 U) when compared with those with SOAG (4.1 ± 1.1 U; p = 0.004). Serum 2-agAAb adrenergic activity did not differ between the cohorts [POAG (4.5 ± 1.5 U); SOAG (4.6 ± 2.1 U; p=0.458)]. No correlation of the beating rates were observed between serum and AH samples for group and subgroup analyses. CONCLUSION: The detection of 2-agAAb in systemic and local circulations supports the hypothesis of a direct functional impact of these agAAbs on ocular G-protein coupled receptors. The high prevalence of 2-agAAb in serum and AH samples of patients with POAG or SOAG suggests a common role of these AAbs in the etiopathogenesis of glaucoma, independent of open-angle glaucoma subtype.
Department of Ophthalmology, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany.
Full articleClassification:
2.6.3 Compostion (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics)
3.7 Biochemistry (Part of: 3 Laboratory methods)
3.9 Pathophysiology (Part of: 3 Laboratory methods)
3.10 Immunobiology (Part of: 3 Laboratory methods)
