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Abstract #95007 Published in IGR 22-2
The potential role of long noncoding RNAs in primary open-angle glaucoma
Zhang F; Zhao Y; Cao M; Jia X; Pan Z; Zhou D; Liu K; Duan XGraefe's Archive for Clinical and Experimental Ophthalmology 2021; 259: 3805-3814
PURPOSE: To identify the potential genes in human trabecular meshwork (TM) related to primary open-angle glaucoma (POAG). METHODS: First, long noncoding RNA (LncRNA) and mRNA expression profiles in TM samples from 4 control subjects and 4 POAG patients were accessed by microarray analyses. Then, twenty lncRNAs were validated by real-time quantitative PCR in the same samples from microarray analyses. Finally, eight highly expressed lncRNAs were further tested by real-time quantitative PCR in TM from 8 normal controls and 19 POAG patients. Expression data were normalized and analyzed using the R software. Pathway analyses were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. RESULTS: A total of 2179 lncRNAs and 923 mRNAs in the TM of POAG patients were significantly upregulated, and 3111 lncRNAs and 887 mRNAs were significantly downregulated. ENST00000552367, ENST00000582505, ENST00000609130, NR_029395, NR_038379, and ENST00000586949 expression levels were significantly higher in the TM from a different cohort of POAG patient than normal controls. CONCLUSION: ENST00000552367, ENST00000582505, ENST000006091- 30, NR_029395, NR_038379, and ENST00000586949 may play essential roles in the development of POAG.
Department of Ophthalmology, The Third Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
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