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Abstract #9671 Published in IGR 5-3
The effect of verapamil on synthesis of extracellular matrix in bovine trabecular meshwork cells
Chen F; Wei H; Zeng YChinese Ophthalmic Research 2003; 21: 392-395
OBJECTIVE: To study the effect of verapamil on synthesis of collagen-I, collagen-III, collagen-IV, fibronectin and hyaluronic acid in bovine trabecular meshwork cells (BTMC). METHODS: The effect of verapamil equal to or less than 0.01 mg/ml on synthesis of collagen-I, collagen-III, collagen-IV, fibronectin, hyaluronic acid in BTMC was examined by immunohistochemistry with computer-aided color image analysis and radioimmunoassay respectively. RESULTS: 0.001, 0.005, 0.01mg/ml verapamil inhibited synthesis of collagen-I, collagen-III, fibronectin in a concentration-dependent manner (p < 0.05), but increased the synthesis of hyaluronic acid (p < 0. 05). 0.005, 0.01mg/ml verapamil could dose-dependently inhibit the synthesis of collagen-IV (p < 0.05), but there was no significant effect on collagen-IV at the 0.001 mg/ml level. CONCLUSIONS: Verapamil may be expected to treat primary open-angle glaucoma (POAG) by inhibiting abnormal deposition of extracellular marix (ECM) in BTMC and subsequently decreasing the resistance to the outflow of aqueous humor. LA: Chinese
Dr. F. Chen, Department of Ophthalmology, Union Hospital, Huazhong Technique University, Wuhan 430022, China
Classification:
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
