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Abstract #9681 Published in IGR 5-3

Aqueous humor dynamics in mice

Aihara M; Lindsey JD; Weinreb RN
Investigative Ophthalmology and Visual Science 2003; 44: 5168-5173

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PURPOSE: To assess aqueous humor dynamics in mouse eyes. METHODS: Aqueous humor dynamics of NIH Swiss White mice were assessed with an injection and aspiration system, using fine glass microneedles. Intraocular pressure (IOP) was measured by a microneedle connected to a pressure transducer. Episcleral venous pressure (EVP) was measured by gradually lowering intracameral pressure until blood reflux into Schlemm's canal was observed. Outflow facility (C) was determined based on constant pressure perfusion measurements obtained at two different IOPs. Aqueous volume (V_a) was determined by direct measurement of aspirated aqueous humor. Aqueous humor production (F_a) was measured by the dilution method with rhodamine-dextran. Conventional and uveoscleral outflow (F_c and F_u, respectively), as well as the turnover rate of aqueous humor, were also calculated. RESULTS: IOP and EVP were 15.7 ± 2.0 and 9.5 ± 1.2 mmHg, respectively (n = 20). F_a was 0.18 ± 0.05 μl/min (mean ± SD; n = 8). C was 0.0051 ± 0.0006 μl/min per mmHg (n = 8). Estimated F_c and F_u were 0.032 and 0.148 μl/min, respectively. F_c was 18% of F_a. F_u was 82% of F_a. V_a was 5.9 ± 0.5 μl (n = 8). The calculated turnover rate of aqueous humor was 2.5%. CONCLUSIONS: The mouse eye has similar aqueous production and aqueous humor turnover rate as the human eye. The presence of both conventional and uveoscleral outflow suggests that the mouse is a useful model system for further investigations of the biology of aqueous dynamics.

Dr. M. Aihara, Hamilton Glaucoma Center and Department of Ophthalmology, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0946, USA

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Classification:

2.6.1 Production (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics)
2.6.2.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics > 2.6.2 Outflow)
2.6.2.2 Uveoscleral (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics > 2.6.2 Outflow)
5 Experimental glaucoma; animal models

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