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Abstract #9705 Published in IGR 5-3
Regulation of antisense oligodeoxynucleotides on expression of Nogo-A in oligodendrocytes in vitro
Peng X; Liu S; Ye JChinese Ophthalmic Research 2003; 21: 485-488
OBJECTIVE: To investigate the effect of antisense oligodeoxynucleotides (ODN) on Nogo-A mRNA expression in oligodendrocytes. METHODS: (1) Oligodendrocytes were obtained by inoculating the optic nerve of two-day old Wistar rats and were identified as oligodendrocytes using immunocytochemical staining. (2) 2, 5, AND 10 μmol/L of Nogo-A ODN and a random sequence ODN were added to the medium in order to observe the effects of Nogo-A ODN on cultured cells. Effects of ODN on the expression of Nogo-A in oligodendrocytes were studied using RT-PCR. RESULTS: (1) On day 3 after inoculation, a few round or fusiform cells with positive GC antibody immunocytochemical staining migrated from the optic nerve tissue. Eleven days later, the coverlips were completely covered by the cells. (2) RT-PCR showed that Nogo-A ODN could significantly and specifically inhibit the expression of Nogo-A after 24 hours' exposure (p < 0.01). Random sequence ODN had no noticeable effect on Nogo-A expresson. CONCLUSIONS: Nogo-A ODN can effectively and specifically inhibit the expression of Nogo-A. This finding establishes the base for further research on the repair of optic nerve injuries. LA: Chinese
Dr. X. Peng, Department of Ophthalmology, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China
Classification:
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
