advertisement
Abstract #99301 Published in IGR 22-4
Discrimination between Healthy Eyes and Those with Mild Glaucoma Damage Using Hemoglobin Measurements of the Optic Nerve Head
Meneses LS; Ciarlini LR; Ayub G; Vasconcellos JPC; Costa VPJournal of Glaucoma 2022; 31: 567-573
PRECIS: The Laguna ONhE, a software that measures the Hb concentration of the optic nerve head from fundus photographs, demonstrated good accuracy in discriminating healthy eyes from eyes with mild glaucoma. PURPOSE: To evaluate hemoglobin concentration of the optic nerve to distinguish between healthy eyes and eyes with mild glaucoma. METHODS: Eyes from patients with mild primary open angle glaucoma (MD > -6▒dB) (n=58) and from healthy subjects (n=64) were selected. Retinal nerve fiber layer thickness (RNFLT) measurements of all eyes were acquired with optical coherence tomography (OCT). Optic disc photographs were also obtained, and the images were analyzed using the Laguna ONhE software, which measures the amount of hemoglobin (Hb) in 24 sectors of the optic nerve head (ONH). The software also calculates the Glaucoma Discriminant Function (GDF), an index that expresses the chance of the ONH being compatible with glaucoma. Areas under the ROC curve (AUCs) and sensitivities at fixed specificities of 90% and 95% of each Laguna ONhE parameter were calculated. RESULTS: The mean RNFLT and vertical C/D ratio of the control and glaucoma groups were 90.0±10.6▒μm versus 66.28±9.85▒μm (P<0.001) and 0.5±0.09 versus 0.65±0.09 (P<0.001), respectively. Total Hb (67.9±4.45 vs. 62.89±4.89, P<0.001) and GDF (11.57±15.34 vs. -27.67±20.94, P<0.001) were significantly higher in the control group. The Hb concentration was also significantly higher in 21 of the 24 sectors in the control group compared to the glaucoma group (P<0.05). The GDF had the largest AUC (0.93), with 79.3% sensitivity at a fixed specificity of 95%. CONCLUSION: Measurements of optic nerve hemoglobin concentration using a colorimetry photographic device demonstrated good accuracy in discriminating healthy eyes from eyes with mild glaucoma. Further studies are need to understand vascular factors implicated in the development of glaucoma.
Department of Ophthalmology, University of Campinas, Campinas, São Paulo, Brazil.
Full article