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Abstract #9975 Published in IGR 5-3
Aqueous dynamic and histological findings after deep sclerectomy with collagen implant in an animal model
Delarive T; Rossier A; Rossier S; Ravinet E; Shaarawy T; Mermoud ABritish Journal of Ophthalmology 2003; 87: 1340-1344
AIM: The use of an animal model to study the aqueous dynamic and the histological findings after deep sclerectomy with (DSCI) and without collagen implant. METHODS: Deep sclerectomy was performed on rabbits' eyes. Eyes were randomly assigned to receive collagen implants. Measurements of intraocular pressure (IOP) and aqueous outflow facility using the constant pressure method through cannulation of the anterior chamber were performed. The system was filled with BSS and cationized ferritin. Histological assessment of the operative site was performed. Sections were stained with hematoxylin and eosin and with Prussian blue. Aqueous drainage vessels were identified by the reaction between ferritin and Prussian blue. All eyes were coded so that the investigator was blind to the type of surgery until the evaluation was completed. RESULTS: A significant decrease in IOP (p < 0.05) was observed during the first six weeks after DSCI (mean IOP was 13.07 (2.95) mmHg preoperatively and 9.08 (2.25) mmHg at six weeks); DS without collagen implant revealed a significant decrease in IOP at weeks 4 and 8 after surgery (mean IOP 12.57 (3.52) mmHg preoperatively, 9.45 (3.38) mmHg at four weeks, and 9.22 (3.39) mmHg at eight weeks). Outflow facility was significantly increased throughout the nine months of follow-up in both DSCI and DS groups (p < 0.05). The preoperative outflow facility (OF) was 0.15 (0.02) μl/min/mmHg. At nine months, OF was 0.52 (0.28) μl/min/mmHg and 0.46 (0.07) μl/min/mmHg for DSCI and DS, respectively. Light microscopy studies showed the appearance of new aqueous drainage vessels in the sclera adjacent to the dissection site in DSCI and DS and the apparition of spindle cells lining the collagen implant in DSCI after two months. CONCLUSIONS: A significant IOP decrease was observed during the first weeks after DSCI and DS. DS with or without collagen implant provided a significant increase in outflow facility throughout the nine months of follow-up. This might be partly explained by new drainage vessels in the sclera surrounding the operated site. Microscopic studies revealed the appearance of spindle cells lining the collagen implant in DSCI after two months.
Dr. T. Delarive, Hôpital Ophtalmique Jules Gonin, Department of Ophthalmology, University of Lausanne, Switzerland
Classification:
12.8.3 Non-perforating (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
