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Abstract #9995 Published in IGR 5-3
Inhibitory effect of tranilast on collagen synthesis in cultured human trabecular meshwork cells
Cao Y; Wei H; Da BChinese Ophthalmic Research 2003; 21: 353-354
OBJECTIVE: To investigate the effect of tranilast on collagen synthesis in cultured human trabecular meshwork cells. METHODS: Cultured passage 3 ≤ 5 human trabecular meshwork cells were treated with 0 μg/ml (control), 12. 5, 25, and 50 μg/ml tranilast and the collagen synthesis of cells was monitored by 3H-proline incorporation and liquid scintillation technique. RESULTS: The rate of 3H-proline incorporation in the groups treated with 12. 5, 25, and 50 μg/ml tranilast were 187 ± 21, 127 ± 18, and 66 ± 12%, respectively. There was significant difference in comparison with the control group (317 ± 48%), and the rate of 3H-proline incorporation into the cultured human trabecular meshwork cells decreased in a dose-dependent manner. CONCLUSIONS: Tranilast could inhibit collagen synthesis in cultured human trabecular meshwork cells. The therapeutical role in treatment of primary open-angle glaucoma should be further investigated. LA: Chinese
Dr. Y. Cao, Department of Ophthalmology, Union Hospital, Huazhong University of Science/Technology, Wuhan 430022, China
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
