advertisement
Abstract #9998 Published in IGR 5-3
Effects of systemic octreotide, local mytomycine-C and local corticosteroids on wound-healing reaction after glaucoma surgery
Akyol N; Demir T; Kukner A; Colakoglu NInternational Ophthalmology 2001; 24: 235-241
PURPOSE: To determine and compare the effectiveness of octreotide, mitomycin-C and corticosteroids on wound-healing reaction after glaucoma surgery. METHODS: Full thickness scleral trephination was carried out by the same surgeon on tour groups of six rabbits. A sponge soaked in mytomicin-C was applied subconjunctivally in group 1 before trephination. Group 2 received corticosteroid drops t.i.d. topically for 14 days. Group 3 received subcutaneous octreotide injections t.i.d. for 14 days. The control group (group 4) was not given any drug that may interfere with wound healing. All groups received gentamycine drops t.i.d. for seven days. The rabbits were sacrificed on the 14th day and the trephination area with overlying conjunctiva was excised. The samples were prefixed with glutaraldehyde, dehydrated and embedded in Araldite Cy 212. Ten semi-thin sections stained with toluidin blue were analyzed for each group. Fibroblast and macrophage counts were performed on the surgical site and subconjunctival area. RESULTS: Intensive fibroblastic activity, increased number of vessels and active macrophages were only observed in group 4. The fibroblast and macrophage densities in this group were significantly higher than the other three groups in which wound healing was modulated (p < 0.001). Mean number of fibroblasts in group 1 was also significantly less than the ones of groups 2 and 3 (p < 0.01). Macrophage densities were similar in groups 1, 2 and 3. No statistical significance was found between groups 2 and 3 with regard to fibroblast and macrophage densities. CONCLUSIONS: Octreotide reduced wound-healing reaction in a similar fashion to corticosteroids or mitomycin-C. These initial results seem promising.
Dr. N. Akyol, Ophthalmology Department, Firat University, School of Medicine, Firat Tip Merkezi Elazig, Turkey
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
