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Abstract #11925 Published in IGR 7-1
A short duration transient ischemia induces apoptosis in retinal layers: An experimental study in rabbits
Oz O; Gurelik G; Akyurek N; Cinel L; Hondur AEuropean Journal of Ophthalmology 2005; 15: 233-238
PURPOSE: To investigate retinal cell apoptosis in an experimental transient, short duration ocular ischemia model. METHODS: An experimental ischemia model, which simulates creating temporary high intraocular pressure to control intraocular bleeding during pars plana vitrectomy, was set up. Rabbits were randomly divided into three groups. Group 1 was the control group. In Group 2, intraocular pressure was increased to 97 mmHg for 5 minutes. In Group 3, intraocular pressure was increased to 97 mmHg for 10 minutes. After 24 hours, terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick-end labeling assay was used to detect retinal apoptosis in rabbit eyes. Only nuclear staining in retinal cells was counted. RESULTS: Groups with 5 minutes and 10 minutes of ischemia showed significantly higher amount of ganglion cell layer apoptosis when compared with the control group (p < 0.05). Light microscopy and standard hematoxylin-eosin did not show any significant damage in the retina cells. CONCLUSIONS: Apoptotic cell death in the retinal cell layers occurs in temporary ischemia-reperfusion as early as 5 and 10 minutes duration.
Dr. O. Oz, Department of Ophthalmology, Mersin University, Mersin, Turkey
Classification:
5 Experimental glaucoma; animal models
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
