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Abstract #12315 Published in IGR 7-2
Variation of four amino acids in vitreous body after negative pressure suction in rabbits
Zhao H; Huang Y; Yang H; Dou HChinese Ophthalmic Research 2005; 23: 183-185
OBJECTIVE: To study the influence of acute intraocular pressure (IOP) elevation on 4 excitatory amino acids levels in vitreous body during laser in situ keratomileusis (LASIK). METHODS: IOP was rapidly increased to 65 mmHg for 45 seconds and 3 minutes respectively by scleral suction on rabbit eyes of different time groups during LASIK. Vitreous body were collected on the instance of operation and in day 7,10,14 and 28 in experimental rabbits and normal control rabbits. Amino acid levels of vitreous body were analyzed with high-pressure liquid chromatography. RESULTS: No significant difference was found in glumate in high IOP for 45 seconds group and control group. Increase of glumate in high IOP for 3 minutes group was more than that of 45 seconds group (t = 3.541, P < 0.05) and reached the peak value of 39.569 9 μg/mL at day 10 (t = 5.981 4, P < 0.01). No ASP and GLY were detected in the vitreous body. In high IOP for 3 minutes group, release of GABA was also increased with the increasing of glutamate and showed a positive relation between them (r = 0.856 9, P < 0.05). No apparent difference was found in 4 amino acids levels in vitreous body between the only laser-treated eyes and the control eyes. CONCLUSION: Acute IOP elevation dose not induce significant changes of 4 amino acids levels in vitreous body after negative suction for 45 seconds during LASIK. Prolonged negative suction time can increase the release of glutamate. LA: Chinese
Dr. H. Zhao, Department of Ophthalmology, General Hospital of PLA, Ophthalmic Center of Chinese PLA, Beijing 100853, China
Classification:
8.4 Refractive surgical procedures (Part of: 8 Refractive errors in relation to glaucoma)
