advertisement
Abstract #14503 Published in IGR 8-4
Anti-apoptosis effect of bcl-xl on retinal ganglion cells by ultrasound-mediated microbubble destruction
Li W; Liu S; Wang Z-GChinese Journal of Medical Imaging Technology 2006; 22: 1147-1150
OBJECTIVE: To assess the effect of bcl-xl gene on N-methyl-D-aspartate (NMDA)-induced apoptosis on cultured retinal ganglion cells (RGCs) by ultrasound-mediated microbubble destruction. METHODS: RGCs were cultured in 24-well plates. NMDA-induced apoptosis in cultured RGCs was established. The experiment was divided into three groups: control, NMDA treatment and bcl-xl transfection+NMDA treatment group (bcl-xl gene was transfected into RGCs 48 h by ultrasound-mediated microbubble destruction before NMDA-induced apoptosis was established). The expression of bcl-xl protein in transfected and non-transfected RGCs was assessed by immunohistochemistry assay. The morphotic character of RGCs was revealed by acridine orange and ethidium bromide staining. DNA fragment was detected by agarose gel electrophoresis. RESULTS: The expression of bcl-xl protein in transfected and non-transfected RGCs was different. Lots of apoptotic bodies was found in NMDA treatment group, less were found in the other two groups. Representative DNA fragment was detected in the NMDA treatment group. CONCLUSION: Transfection of bcl-xl has anti-apoptosis effect on cultured RGCs from apoptosis induced by NMDA with ultrasound-mediated microbubble destruction. It may be a promising gene therapy for retinal and optic nerve diseases. LA: Chinese
Dr. S. Liu, Department of Ophthalmology, Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China
Classification:
11.8 Neuroprotection (Part of: 11 Medical treatment)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
11.9 Gene therapy (Part of: 11 Medical treatment)
