advertisement
Abstract #14540 Published in IGR 8-4
Concentrations of mitomycin C in rabbit corneal tissue and aqueous humor after topical administration
Song J-S; Kim J-H; Yang M; Sul D; Kim H-MCornea 2006; 25: S20-S23
PURPOSE: To study the aqueous and corneal pharmacokinetics of mitomycin C (MMC) after single topical administration to the central cornea and to evaluate the effects of different concentrations and different application times on the aqueous concentration of MMC. METHODS: Mechanical epithelium debridement of the central 7.5 mm of the cornea was performed in New Zealand white rabbits, and a sponge soaked in 0.02% MMC solution was placed on the denuded corneal stroma for 2 minutes. Aqueous fluid and central corneal tissues samples were taken at 0.5, 1, 2, and 3 hours thereafter. MMC concentration of the samples was analyzed by high-performance liquid chromatography and evaluated at different exposure times (range: 15-120 seconds) and concentrations of applied MMC (range: 0.005%-0.04%). RESULTS: Peak corneal concentration was 3.728 ± 2.547 μg/g at 30 minutes after topical administration. Maximum aqueous concentration was 0.380 ± 0.038 μg/mL at 1 hour after topical application. The aqueous concentration of MMC increased in a dose-dependent manner with increasing exposure time and application concentration. Aqueous MMC concentration increased at a higher rate with change of applied concentration than with exposure time. CONCLUSION: Good penetration of MMC through central bare cornea may be noxious to endothelial cells. Reducing concentration or decreasing exposure time seems a good modality to reduce potential MMC toxicity.
Dr. H.-M. Kim, Department of Ophthalmology, Anam Hospital, Korea University College of Medicine 126-1, 5ka Anam-dong, Seongbuk-gu, Seoul 136-701, Korea
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
5 Experimental glaucoma; animal models
