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Abstract #15588 Published in IGR 1-3
The effect of mitomycin C after long-term storage on human Tenon's fibroblast proliferation
Hu D; Chen PP; Oda DJournal of Glaucoma 1999; 8: 302-305
PURPOSE: To investigate the effect of mitomycin C (MMC) after long-term storage on proliferation of human Tenon's fibroblasts in vitro. METHODS: Human Tenon's fibroblasts in tissue culture were exposed for five minutes to MMC (0.4 mg/ml) that was either freshly prepared or had been stored for as long as 18 months at either 4°C or -20°C. The MTT colorimetric assay was used to determine the inhibition of proliferation as measured indirectly by mitochondrial activity. RESULTS: The inhibition rate was 88% using fresh MMC, and declined to a mean of 73% when using MMC that had been stored for as long as 18 months at 4°C; this decrease was not statistically significant. The mean inhibition for MMC stored at -20°C was 68%, and this was significantly less than inhibition with fresh MMC. Inhibition did not vary significantly with MMC after different storage times. CONCLUSIONS: MMC continues to have strong in vitro antiproliferative effects when stored for as long as 18 months at 4°C or -20°C. A significant decline in potency compared with fresh MMC occurs when MMC is stored at -20°C.
Dr. P.P. Chen, Department of Ophthalmology, Box 356485, University of Washington, Seattle, WA 98195-6485; USA
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)