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Abstract #20579 Published in IGR 10-1
High-resolution imaging of retinal cells in the living eye
Paques M; Simonutti M; Roux MJ; Bellman C; Lacombe F; Grieve K; Glanc M; LeMer Y; Sahel J-AEye 2007; 21: S18-S20
During diseases such as age-related macular degeneration, glaucoma, or retinal degeneration, visualizing and counting retinal cells would be of interest to diagnose early stages and to determine the short-term progression rate of these conditions. This is of major interest to optimize neuroprotective/regenerative therapies in these slowly progressive diseases. Such cellular imaging is already feasible in the eye of laboratory animals. In the near future, it is likely that visualization of individual neuronal cells in humans will become a routine clinical procedure, thanks to continuous technological improvements in optical imaging technologies. In mice, commercially available confocal scanning laser ophthalmoscope (cSLO) allows imaging of axons, capillaries, as well as dye-labelled cells (such as gfp-expressing cells). In humans, commercially available optical coherence tomography (OCT) allows routine imaging of retinal layering, but not of individual cells. Several research groups (including ours) are working in the highly competitive area of retinal cellular-imaging in humans. Different systems of adaptive optics, SLO, and OCT (in spectral domain a-mode or en face mode) and second harmonic generation imaging are under evaluation. These techniques have demonstrated cellular-level imaging capabilities of photoreceptors, of the nerve fibre layer and of capillaries. The main challenges that these systems face are biological (determination of the origin of the signal), medical (the clinical pertinence of the information) and technical (optimization of hardware, development of user interface).
Dr. J.-A. Sahel, INSERM, Universite Pierre et Marie Curie6, UMR-S0592, Paris, France
Classification:
6.9.1.1 Confocal Scanning Laser Ophthalmoscopy (Part of: 6 Clinical examination methods > 6.9 Computerized image analysis > 6.9.1 Laser scanning)
6.9.5 Other (Part of: 6 Clinical examination methods > 6.9 Computerized image analysis)
6.9.2.2 Posterior (Part of: 6 Clinical examination methods > 6.9 Computerized image analysis > 6.9.2 Optical coherence tomography)
