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Abstract #22017 Published in IGR 10-4
Protection of autoimmunity induced by copolymer-1 on optic nerve: Experiment with rat glaucoma models
Li X; Qian S-H; Sun X-HZhonghua Yi Xue Za Zhi 2008; 88: 2152-2154
OBJECTIVE: To investigate whether eopolymer-1 (Cop-1) a synthesized analogue of myelin basic protein, can protect the retina ganglion cells (RGCs) and possible mechanism thereof. METHODS: Vortex veins of rats were ligated to induce an increase of intraocular pressure (IOP) so as to establish glaucoma models. One hundred-six rat models were randomly divided into 2 equal groups: Cop-1 Group undergoing subcutaneous injection of 200 μg Cop-1, and phosphate buffered solution (PBS) Group undergoing PBS injection as controls. Fluorogold was used to retrogradely label the RGCs. 10, 17, 24, and 31 days after the immunization some rats were killed with their eyeballs taken out. The fluorogold positive spots were calculated. Anti-rat T cell receptor (TCR) monoclonal antibody was used to mark T cells in the retina. RESULTS: 17, 24, and 31 days after die immunization, the RGC density of the Cop-1 Group were (2617 ± 17)/mm2, (2588 ± 206)/mm2, and (2394 ± 15)/mm2 respectively, all significantly higher than those of the PBS Group [(2357 ± 37)/mm2, (2277 ± 340)/mm2, and (2129 ± 17)/mm2 respectively, all P < 0.05]. 10, 17, 24, and 31 days after the immunization the numbers of T cells in the retina of Cop-1 Group were (11.3 ± 2.8)/mm2, (36.7 ± 5.2)/mm2, (33.9 ± 3.0)/mm2, and (21.4 ± 5.9)/mm2, all significantly higher than those of PBS Group (4.7 (±) 3.6)/ mm2, (19.7 ± 2.4)/mm2, (15.3 ± 4.0)/mm2, and (13.3 ± 4.4)/mm2 respectively, all P < 0.01). CONCLUSION: Cop-1 protects RGCs. The injury of RGCs induced by increased IOP results in congregation of T cells. Cop-1 increases the number of T-lymphocyte cells congregating in the retina, which may be related to its neuroprotection. LA: Chinese
Dr. S.-H. Qian, Department of Ophthalmology, Eye, Nose, and Throat Hospital, Fudan University, Shanghai 200031, China. qsh2304@sohu.com