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Abstract #22044 Published in IGR 10-4
Comparison of damage of benzalkonium chloride and timolol to human lens epithelial cell
Xiang L; Liu Y; Zhang JChinese Ophthalmic Research 2008; 26: 658-662
OBJECTIVE: The apostolic of the human lens epithelial cell has been implied to be the reason for occurrence of the cataract. Some clinical trials showed that benzalkonium chlorid, as one of the composition of timolol maleate, brings some adverse effects on human eye tissue. This research was to investigate the effects of benzalkonium chloride and timolol maleate on human lens epithelial cells (LECs). METHODS: Human LECs strains (SRA01/04) was cultured in DMEM containing bovine serum. Different concentrations of benzalkonium chloride and timolol was added into medium. The morphological changes were observed under the inverted contrast phase microscope. The activity of cultured LECs were measured by MTT, and cell-free culture supernatants were collected for the detection of prostaglandin E(2)(PEG(2)) and interleukin 1α (IL-1α) by ELISA. RESULTS: LECs with both benzalkonium chloride and timolol detached from the culture dish and died within 24 hours. LECs could be disintegrated and elongated in 5 x 10-2 mg/mL of timolol and 1 x 10-4 mg/mL benzalkonium chloride. The activity of cultured cell was enhanced with the increase of diluting times of benzalkonium chloride and timolol. The amounts of PEG(2) and IL-1α depended on the dose of the drugs. When timolol was at 5 mg/mL, it killed the cells directly and its secretion of PEG(2) and IL-1α was almost stopped. But when it was diluted from 5 x 10-1 to 5 x 10-2 mg/mL, survival cells increased. Although at the dilution of 0.25 mg/mL, the secretion of PEG(2) and IL-1α showed the highest level (P < 0.05). Benzalkonium chloride, on the other hand, showed continuously chronic inflammatory stimulate: the secretion of PEG(2) and IL-1α elevated even if it was diluted, and peaked at 1 x 10-4 mg/mL (P < 0.05). CONCLUSION: Benzalkonium chloride induces the damage of human LECs, and it strongly stimulates the cultured LECs to secrete the inflammatory mediators. LA: Chinese
Dr. L. Xiang, Department of Ophthalmology, West China Medical Center, Sichuan University, Chengdu 610041, China. yourshirley99@163.com
