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Abstract #22423 Published in IGR 10-4
Structural observation of the anterior chamber angle in CYP1B1-null mice
Jiang FG; Cui L; Liu XWZhonghua Yan Ke Za Zhi 2008; 44: 418-422
OBJECTIVE: To determine the effects of CYP1B1 gene on the structure of the anterior chamber angle in mice. METHODS: Experimental study. Adult CYP1B1-null mice were used, in comparison C57BL/6J mice was used as control. The mice eyes were enucleated and plastic-embedded and the anterior chamber angle was sagittally sectioned in 3 micrometers thickness. The morphology and structure of the eye tissue was studied by light microscopy and transmission electron microscopy. RESULTS: The anterior chamber angle in C57BL/6J mice was well-developed. But in CYP1B1-null mice, a variety of anomalies were found in the these tissues, including hypoplastic and compressed trabecular meshwork, abnormal structure of trabecular cells, a basal lamina extending from cornea over the meshwork, small or absent Schlemm's canal, and long iris process extending from the iris root to the trabecular meshwork. These pathological changes were demonstrated focally and the rest of the angle in CYP1B1-null was normal. CONCLUSIONS: CYP1B1 gene plays an important role in the development of the anterior chamber angle. Deletion of this gene leads to the abnormalities in trabecular meshwork, Schlemm's canal and iris process, which may have some influence on the metabolism and function of the outflow facilities. LA: Chinese
Dr. F.G. Jiang, Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. fgjiang@hotmail.com
