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OBJECTIVE: To investigate the effect of connective tissue growth factor's antisense oligonucleotides (ASODN) on the growth of human Tenon's capsule fibroblasts (HTF) induced by transforming growth factor β-2 (TGF-β-2) in vitro. METHODS: It was a experimental study. HTF was collected from glaucoma patients and cultured. The 5-6 passage was used for experiments. The HTF induced by TGF-β-2 was divided into the following groups: N group: normal HTF; T group: HTF induced by TGF-β-2; A group: CTGF ASODN antisense:5'-TACTGGCGGCGGTCAT-3' encapsulated with liposome; S group: sense 5'-ATGACCGCCGCCAGTA-3' encapsulated with liposome; D group: HTF encapsulated with liposome only. The activity of HTF treated by different concentrations of liposome was detected using methylthianolyldiphenyl tetrazolum bromide (MT) colorimetry. The expression of CTGF was detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry assays. The expression of fibronectin (Fn) was examined by Western blot and immunocytochemistry assays. RESULTS: Liposome-ASODN (A group) significantly (F = 15.25, 204.88, 19.73, 90.00; P < 0.05) inhibit the expression of CTGF and Fn in HTF induced by TGF-β-2 compared with S and D group. However, Liposome alone (T group) has no significant impact in HTF growth compared with T group (t = 0.90, 2.32, 0.75, 2.11; P > 0.05). CONCLUSION: CTGF-ASODN inhibits the CTGF and Fn expression of HTF induced by TGF-β-2, which may delay the formation of scar in glaucoma filtering surgery. LA: Chinese
Dr. Y.Z. Hu, Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China