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Abstract #24514 Published in IGR 11-3
Sustained Release and Permeation of Timolol from Surface-Modified Solid Lipid Nanoparticles through Bioengineered Human Cornea
Attama AA; Reichl S; Mller-Goymann CCCurrent Eye Research 2009; 34: 698-705
PURPOSE: The aim of the study was to formulate and evaluate surface-modified solid lipid nanoparticles sustained delivery system of timolol hydrogen maleate, a prototype ocular drug using a human cornea construct. MATERIALS AND METHODS: Surface-modified solid lipid nanoparticles containing timolol with and without phospholipid were formulated by melt emulsification with high-pressure homogenization and characterized by particle size, wide-angle X-ray diffraction, encapsulation efficiency, and in vitro drug release. Drug transport studies through cornea bioengineered from human donor cornea cells were carried out using a modified Franz diffusion cell and drug concentration analyzed by high-performance liquid chromatography. RESULTS: Results show that surface-modified solid lipid nanoparticles possessed very small particles (42.9 ± 0.3 nm, 47.2 ± 0.3 nm, 42.7 ± 0.7 nm, and 37.7 ± 0.3 nm, respectively for SM-SLN 1, SM-SLN 2, SM-SLN 3, and SM-SLN 4) with low polydispersity indices, increased encapsulation efficiency (> 44%), and sustained in vitro release compared with unmodified lipid nanoparticles whose particles were greater than 160 nm. Permeation of timolol hydrogen maleate from the surface-modified lipid nanoparticles across the cornea construct was sustained compared with timolol hydrogen maleate solution in distilled water. CONCLUSIONS: Surface-modified solid lipid nanoparticles could provide an efficient way of improving ocular bioavailability of timolol hydrogen maleate.
Dr. A.A. Attama, Institut fr Pharmazeutische Technologie, Technische Universitt Carolo-Wilhelmina zu Braunschweig, Braunschweig, Germany
Classification:
11.16 Vehicles, delivery systems, pharmacokinetics, formulation (Part of: 11 Medical treatment)
