advertisement
Abstract #24616 Published in IGR 11-4
The neuroprotective effect of extact of ginko biloba on retinal ganglion cells after retinal ischemia-reperfusion
Li Y; Ma K; Xu LChinese Ophthalmic Research 2009; 27: 660-663
Objective: Loss of retinal ganglion cells (RGCs) is a dominant pathological feature after elevation of intraocular pressure(IOP). Our previous study showed that ginkgo biloba extract has a neuroprotective effect on RGCs. Present study was to investigate the effect of extact of ginko biloba on RGCs survival after acute ocular hypertention-induced retinal ischemia-reperfusion injury. Methods: Retinal ischemia-reperfusion models were induced by infusing normal saline solution into anterior chamber to elevate IOP to 110mmHg for one hour and then reducing the IOP to normal reading to allow reperfusion in the right eyes of 60 SD rats. The normal fellow eyes were as controls. 5 ml/kg of normal saline solution (NS), 1% erigeron breviscapus(EB), 0.25% EGb761,1% EGb761,4% EGb761 was administered via lavaging at 2 hour after injury and once a day after operation for 28 days respectively in different groups. RGCs were retrogradely labeled by injection of 3% fluorogold into both side of superior colliculus at the 23th day after injury. The rats were sacrificed and retinas were collected at the 28th day after injury. Survival rate of RGCs was calculated at the ratio of RGCs density between right eyes and left eyes. Animal experiment followed the Standard of Association for Research in Vision and Ophthalmology. Results The mean survival rate of RGCs was 66.58% (plus or minus) 6.83%, 75.62% (plus or minus) 6.9%, 74.92% (plus or minus) 6.78%, 76.57% (plus or minus) 7.86%, 79.87% (plus or minus) 7.49% in NS group, 1% erigeron breviscapus group, 0.25%, 1% and 4% EGb761 group respectively, indicating significant difference among different groups(F = 5.13, P = 0.001). Compared with NS group, the survival rate of RGCs was significantly elevated in 1% erigeron breviscapus (EB) group, 0.25% EGb761 group, 1% EGb761 group and 4% EGb761 group(P < 0.05). No significant difference was found among different doses of EGb761 groups(P > 0.05) or between 1% EB group and different doses of EGb761 groups(P > 0.05). Conclusion EGb761 has neuroprotective effect on RGCs in acute IOP induced retinal ischemia-reperfusion injury. LA: Chinese
Y. Li. Department of Ophthalmology, Beijing Tongren Hospital, Beijing 100005, China.
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
11.8 Neuroprotection (Part of: 11 Medical treatment)
11.15 Other drugs in relation to glaucoma (Part of: 11 Medical treatment)
