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Abstract #24716 Published in IGR 11-4
Proteomic study of DBA/2J mice retina: Down-regulation of Integrin (beta)7 correlated with retinal ganglion cell death
Kanamoto T; Ue T; Yokoyama T; Souchelnytskyi N; Kiuchi YProteomics 2009; 9: 4962-4969
To identify and determine the function of the proteins associated with the death of retinal ganglion cells (RGCs) in DBA/2J mice, an animal model of glaucoma, retinas of DBA/2J mice, were analyzed by proteomics at 5-, 7-, and 11-months-of-age. The proteins showing significant alterations were selected for identification by MS and 18 proteins were differentially expressed and the identified proteins included cell membrane receptors and proteins associated with intracellular signaling pathways. Among of identified proteins, the expression of Integrin (beta)7 at 7-months-of-age was decreased by about 89% of that at 5-months-of-age. Integrin (beta)7 was expressed in the RGCs. The effect of glutamate toxicity on the expression pattern of Integrin (beta)7 in a RGC line was also investigated and the glutamate-induced death of RGC was inhibited by the RNA knockdown of Integrin (beta)7. Our data showed also that the expression of 18 proteins in the DBA/2J was significantly altered in DBA2 mice and downregulation of Integrin (beta)7 may have a protective effect on glutamate-induced death of RGCs.
T. Kanamoto. Department of Ophthalmology and Visual Science, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8551, Japan. tkana@Hiroshima-u.ac.jp
Classification:
3.12 Proteomics (Part of: 3 Laboratory methods)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
3.6 Cellular biology (Part of: 3 Laboratory methods)
