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Abstract #26632 Published in IGR 12-3
Establishment of chronic glaucoma animal model by combination of aspiration of aqueous with photocoagulation of anterior chamber angle
Wang Y; Wu L-L; Wang D-MChinese Ophthalmic Research 2010; 28: 481-485
Background: Many methods to establish an animal model of glaucoma in rat have been reported. But these methods are thought to have some disadvantages. A newly developed method for raising intraocular pressure was created by the aspiration of aqueous humor combined with the laser photocoagulation at the limbus in mouse recently. Objective: The aim of this study was to develop an inducible model of the chronic glaucoma with optic neuropathy induced by intraocular hypertension in rats. Methods: The ocular hypertension model was established in the right eyes of 80 Wistar rats under the anesthetization of ketamine and xylazine. The anterior chamber was flattened by the aspiration of aqueous humor with a microneedle, and then a krypton laser with wavelength of 532 nm was used to burn the corneoscleral limbus and episcleral veins of right eyes at 80 Wistar rats, and the left eyes worked as controls. Tono-Pen XL tonometer was used to measure IOP at the regular time point at 1 day, 3 days, 1 week and weekly for 8 weeks after surgery. The retina and optic nerve samples were obtained to evaluate the histopathological change of animal model by hematoxylin-eosin staining and Nissl staining. The use of animal followed the Standard of Association for Research in Vision and Ophthalmology. Results: The mild inflammation response was found in 1-3 days after operation. The IOP was significantly higher from 1 day through 28 days after operation in the right eyes compared with the left eyes (all P < 0.01). The IOP was enhanced in 1 day through 21 days in postoperation in comparison with preoperation (P < 0.01). No obvious change was found in IOP in the left eyes in different time points (all P > 0.05). Hematoxylin-eosin staining exhibited the abnormal structure of the anterior chamber angle in the right eyes after operation, and Nissl staining showed that Nissl bodies were decreased in retinal ganglion cells in the right eyes. The numbers of retinal ganglion cells in the right eyes were significantly declined in comparison with the left eyes (P < 0.05). Conclusion: The rat model of chronic elevation of IOP can be induced by aspiration of aqueous combined with a laser injury to the corneoscleral limbus and episcleral veins.LA: Chinese
Y. Wang. Peking University Eye Center, Peking University Third Hospital, Beijing 100191, China.
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
