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Abstract #46969 Published in IGR 13-3
Vitamins A, E, B12 and folate levels in different types of glaucoma
Belmonte A; Tormo C; Lopez N; Villalba C; Fernandez C; Hernandez FClinical Chemistry and Laboratory Medicine 2011; 49: 816
Background. The relationship between the increase in intraocular pressure and nutritional and environmental features in patients affected with glaucoma could suggest a role of oxidative stress in the etiology of this pathology. To address this hypothesis, we will determine the levels of the antioxidative vitamins A, B12, E and folate in 48 patients affected with primary open-angle glaucoma (POAG) and 13 patients with normal tension glaucoma (NTG). Results were compared with the data obtained from 78 healthy controls (C). Methods. Patients were evaluated and selected in the Ophthalmology Department. Serum samples were collected. Vitamins A and E were determined by High Pressure Liquid Chromatography (Bio-Rad). Vitamin B12 and folate were determined by solidphase competitive chemoluminiscence immunoassay (Architect, Abbott). ANOVA test was used for statistical treatment of data. Results. The levels were as follows: vitamin A (POAG: 39.7+/-13.2 ug/dL; NTG: 30.4+/-15.8 ug/dL; C: 37.9+/-12.0 ug/dL) (p=0.061); vitamin E (POAG: 1050.4+/-330.5 ug/dL; NTG: 739.9+/-288.0 ug/dL; C: 1008.7+/-232.5 ug/dL) (p=0.001); vitamin B12 (POAG: 404.3+/-198.2 pg/mL; NTG: 471.7+/-177.6 pg/mL; C: 425.7+/-137.7 pg/mL) (p=0.385) and folate (POAG: 6.7+/-3.6 pg/ mL; NTG: 5.9+/-3.4 pg/mL; C: 5.7+/-2.8 pg/mL) (p=0.173). Conclusions. Both pathologies show important differences in the homeostasis of the different vitamins. Patients with POAG show a significant increase in vitamins A and E levels when compared with NTG patients. Indeed, this patients (NTG) show even lower levels of both vitamins than healthy controls. Vitamin deficiency in NTG patients could trigger this pathology trough an increase in oxidative stress.
A. Belmonte. Clinical Laboratory and, General University Hospital, Elche, Spain.
