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Abstract #46970 Published in IGR 13-3
Vascular Damage's markers in glaucoma patients
Lopez N; Villalba C; Tormo C; Belmonte A; Fernandez C; Hernandez FClinical Chemistry and Laboratory Medicine 2011; 49
Background. Recently, vascular risk factors show an increasing significance due to its association with glaucoma pathogenesis. For this reason, two blood markers of vascular damage, homocysteine and endotheline-1 (ET-1), have been determined in 48 patients affected with primary open-angle glaucoma (POAG) and in 13 patients affected with normal tension glaucoma (NTG). Results from glaucoma patients were compared with the data obtained in 78 healthy controls (C). Methods. Patients were selected and evaluated in the Ophthalmology Department. Cold EDTA plasma and serum samples were collected. Homocysteine levels were determined by solid-phase competitive chemoluminiscence enzyme assay (Architect, Abbott). Enzyme immunoassay (Biomedica) was used to determine ET-1 levels. ANOVA and Pearson tests were used for statistical treatment of data. Results. The levels of the two markers in the different groups of study were as follows: Homocysteine (POAG: 7.56+/-2.05 umol/L; NTG: 6.40+/-2.47 umol/L; C: 5.94+/-2.57 umol/L) (p=0.002) and ET-1 (POAG: 5.4+/-5.3 fmol/mL; NTG: 4.1+/-2.9 fmol/mL; C: 2.9+/-2.4 fmol/mL) (p=0.002). In the NTG group, a statistically significant correlation between both markers was found (r=0.928; p=0.028). However, this correlation was not found in the POAG and control groups. Conclusions. An increase in the levels of both markers, homocysteine and ET-1, is found in patients affected either with openangle glaucoma and normal tension glaucoma, indicating vascular damage in both pathologies. However, correlation studies suggest different mechanisms implicated in the appearance and evolution of each type of glaucoma.
N. Lopez. Clinical Laboratory, General University Hospital, Elche, Spain.
Classification:
3.7 Biochemistry (Part of: 3 Laboratory methods)
6.11 Bloodflow measurements (Part of: 6 Clinical examination methods)
