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Abstract #6300 Published in IGR 3-2
Detection of the expression of p53 gene on retinal ganglion cells of rabbit eyes with intraocular hypertension
Rongjiang L; Jian G; Yehong ZChinese Ophthalmic Research 2001; 19: 208-210
OBJECTIVE: To detect the expression on p53 gene in retinal ganglion cells (RGCs) of rabbit eyes with different degrees of intraocular hypertension. METHODS: Intraocular pressure (IOP) was elevated by intracameral injection of 0.100.15 ml of 2% methylcellulose. Animals were grouped up by different values of IOP. The rabbit eyes were enucleated, fixed, paraffin-embedded and cut into serial 6-μm thin sections. For histological evaluation, the slides were stained with DAB chromogen (conducting an immuohistochemical experiment), and were examined by light microscopic and automatic image analysis. RESULTS: The number of RGCs with a positive reaction for p53 monoclonal antibody was statistically higher in the experimental eyes than in their control fellow eyes (p < 0.01), and apoptosis was mainly found in the early stage of research. CONCLUSIONS: This research presents a new alternative view of apoptosis in experimental glaucoma. (According to former reports, apoptosis was mainly found in the advanced stage of the patients with POAG.) LA: Chinese
Dr L. Rongjiang, Zhongshan Ophthalmic Centre, Sun Yat-sen University of Medical Sciences, Zhongshan 510060, China
Classification:
1.2 Population genetics (Part of: 1 General aspects)
5 Experimental glaucoma; animal models