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Abstract #6337 Published in IGR 3-2
Comparison of nerve fiber layer thickness between optical coherence tomography and histomorphometry in glaucomatous monkey eyes
Huang L; Schuman J; Wang NChinese Journal of Ophthalmology 2001; 37: 188
OBJECTIVE: To correlate the retinal nerve fiber layer (NFL) thickness measured by optical coherence tomography (OCT) in vivo with histomorphometric measurements. METHODS. Four laser-induced glaucomatous eyes of four cynomolgus monkeys were studied by OCT scanning a circular area 2.4 mm in diameter around the optic nerve head. The four eyes had early or advanced glaucoma. After the final OCT measurement, the monkeys were sacrificed and the eyes enucleated. Routine serial section preparations were made, stained with hematoxylin and eosin, and the NFL thickness of 526 sections measured with the aid of a computerized image analysis system was compared with that at the points coincident with the OCT scans. RESULTS: The mean OCT and histomorphometric NFL thickness in glaucoma were 72.2 ± 54.5 and 61.1 ± 44.2 μm, after the correction for dehydration 15%, 70.3 ± 51.1 μm, respectively. Student's t test showed that the difference was not significant (t = 1.895, p > 0.05). CONCLUSIONS: The NFL thickness measured by OCT in vivo correlates well with that by histomorphometry, and the measurement is accurate.LA: Chinese
Dr L. Huang, Shenzhen Eye Hospital, Shenzhen 518001, China
Classification:
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
6.9.2 Optical coherence tomography (Part of: 6 Clinical examination methods > 6.9 Computerized image analysis)
