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Abstract #6489 Published in IGR 3-2
Effects of tetramethylpyrazine on prostaglandin E2- and prostaglandin E2 receptor agonist-induced disruption of blood-aqueous barrier in pigmented rabbits
Katagawa K; Hayasaka S; Nagaki Y; Watanabe KJapanese Journal of Ophthalmology 2001; 45: 227-232
PURPOSE: To evaluate the effect of tetramethylpyrazine on the elevation of the aqueous flare and intraocular pressure (IOP) induced by prostaglandin (PE) E2 and PGE2 receptor (EP) agonists. METHODS: PGE2 or EP agonists (11-deoxy PGE1, EP2 agonist; 17-phenyl trinos PGF2, EP1 and EP3 agonist; or sulprostone, EP1 and EP3 agonist), 25 μg;ml, were transcorneally administered to pigmented rabbits. Animals were pretreated with tetramethylpyrazine intravenously (10 or 30 mg/kg) or topically (0.1% solution). Aqueous flare was measured using a laser flare-cell meter, and the intensity was expressed as the area under the curve (AUC). IOP was measured using a noncontact tonometer. RESULTS: After administration of PGE2, aqueous flare and IOP increased and then gradually decreased. The AUC of eyes pretreated with tetramethylpyrazine, 10 or 30 mg/kg intravenously, or topical 0.1% solution, was significantly smaller than that of controls. The mean Δ IOP of eyes pretreated with tetramethylpyrazine, 30 mg/kg intravenously, was significantly lower than that of controls. After administration of 11-deoxy PGE1, aqueous flare increased and then gradually decreased. 17-Phenyl trinor PGE2 and sulprostone did not disrupt the blood-aqueous barrier. The AUC of eyes pretreated with tetramethylpyrazine, 10 or 30 mg/kg intravenously, before 11-deoxy PGE1 application was significantly smaller than that of controls. CONCLUSION: These results indicated that tetramethylpyrazine inhibited PGE2- or 11-deoxy PGE1-induced elevation of aqueous flare and IOP.
Dr K. Kitagawa, Department of Ophthalmology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama-Ken 930-0194, Japan
Classification:
11.4 Prostaglandins (Part of: 11 Medical treatment)
