advertisement
Abstract #6526 Published in IGR 3-2
Increase of free oxygen radicals in aqueous humour induced by selective Nd:YAG laser trabeculoplasty in the rabbit
Guzey M; Vural H; Satici A; Karadede S; Dogan ZEuropean Journal of Ophthalmology 2001; 11: 47-52
PURPOSE: To investigate the impact of selective Nd:YAG laser trabeculoplasty on free oxygen radicals and antioxidant enzymes of the aqueous humor in the rabbit. METHODS: One eye of 18 rabbits was subjected to 360° selective laser trabeculoplasty (LT) with a frequency-doubled Nd:YAG laser (532 nm). The anterior chamber aqueous humor was aspirated three, 12 hours and one, three, seven, and ten days after treatment. Lipid peroxide (LPO) and glutathione S transferase (GST) levels and superoxide dismutase (SOD) activities of aqueous humor were measured. RESULTS: Concentrations of LPO in the aqueous humor of the treated eyes were significantly higher than the untreated eyes until the seventh day. Aqueous SOD activity significantly decreased three hours after LT and remained low until day 7. Aqueous GST levels were significantly decreased between 12 hours and seven days after the LT. CONCLUSIONS: Selective LT was followed by an immediate increase in the aqueous humour LPO concentration and decreases of SOD and GST in the rabbit, probably due to photovaporization and photodisruption caused by the frequency-doubled Nd:YAG laser. The increased aqueous LPO levels suggest that free oxygen radicals are formed in the pigmented trabecular meshwork during LT, and may be responsible for the inflammatory complications of this procedure.
Dr M. Guzey, Department of Ophthalmology, Faculty of Agricolture, Harran University, Sanliurfa, Turkey. guzey@turk.net
Classification:
12.4 Laser trabeculoplasty and other laser treatment of the angle (Part of: 12 Surgical treatment)