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Abstract #9670 Published in IGR 5-3
In vitro culture of bovine trabecular meshwork cells by digestion method
Zhang W; Yang X; Li YChinese Ophthalmic Research 2003; 21: 406-408
OBJECTIVE: To look for a culture method for bovin trabecular cells in vitro in order to provide the necessary conditions for the experimental study of primary open angle glaucoma (POAG). METHODS: 0.25% pencreatin and 0.3% collagenase were equally mixed to obtain primary bovine trabecular meshwork cells, and only 0.25% pencreatin for the passage of culture. RESULTS: Stable growth status of bovine trabecular meshwork cells were successful cultured by digestion method in vitro. CONCLUSION: The digestion method can be used for the primary and passage culture of trabecular meshwork cells. LA: Chinese
Dr. W. Zhang, Department of Ophthalmology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China
Classification:
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
