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Abstract #24304 Published in IGR 11-3
The effect of H-1152 on IOP and trabecular meshwork structure in rabbit
Wang Y; Sun J; Cai S; Qiu J; Yu M; Cao G; Liu X; Li XChinese Ophthalmic Research 2009; 27: 453-456
OBJECTIVE: Glaucoma is an optic neuropathy caused by elevated intraocular pressure (IOP). To low IOP is still the major therapeutic approach to this disease. Our study was designed to investigate the effects of Rho-kinase inhibitor, H-1152, on IOP and ultrastructure of trabecular meshwork in rabbits. METHODS: 50 (mu)L of 10 mmol/L H-1152 was topically administered in the right eyes of 6 New Zealand white rabbits and the equal amount of physiology balance solution (PBS) was used at the same way in the left eyes as control. IOP was measured before and after the topical administration of H-1152 using pneumatometer. The ocular inflammatory manifestation was examined by the slim-lamp for the evaluation of adverse effects of H-1152. The morphologic change of rabbits trabecular meshwork was examined under the light and transmission electron microscopy. RESULTS: The IOP was significantly declined from 1 hour through 6 hours after application of 10 mmol/L (50 μL) H-1152, showing a statistically significant difference in comparison with control group(P < 0.05). The maximum decrease scope of IOP was 50.6% at 2 hours after the topical administration of H-1152. Compared with the control groups, IOP reduction was the most dominant in the duration of 1 hour and 3 hours after H-1152 administration. No visible structural alteration was seen in trabecular meshwork tissue under the light microscope. However, the shortening of cells and widening of the extracellular spaces among the beams of the trabecular meshwork were displayed under the transmission electron microscopy in H-1152 group. No obvious adverse effect on eye tissue during the application duration of H-1152. CONCLUSION: H-1152 decrease the IOP in rabbit probably by deterioration of the actin cytoskeleton and cellular adhesions and the expansion of the intercellular spaces in the juxtacanalicular region. The result suggests that H-1152 has the potential effect of IOP control as an anti-glaucoma medication. LA: Chinese
Dr. Y. Wang, School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou 325025, China
Classification:
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
5.3 Other (Part of: 5 Experimental glaucoma; animal models)
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)