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Abstract #20719 Published in IGR 10-1
Expression of GAP-43 in the retina of rabbit subjected to experimental ocular hypertension
Yang X-G; Wang Y-W; Jin W-L; Sun D-J; Hang C; Wang B-RInternational Journal of Ophthalmology 2008; 8: 50-52
AIM: Growth-associated protein-43 (GAP-43) plays an important role in the development and regeneration of central nervous system. In the present study, we examined the alteration of GAP-43 expression in the rabbit retina under experimental persisted ocular hypertension. METHODS: A chronic ocular hypertension model was established in the rabbit by an injection of Carbomer compound solution into anterior chamber. The expressions of GAP-43 in the retina were observed by means of immunohistochemistry at different time points after the ocular hypertension was produced. RESULTS: A moderate expression of GAP-43 was observed in the inner plexiform layer (IPL) of the retina in normal rabbit eyes. Seven days after ocular hypertension, the density of positive reaction products was significantly increased in IPL compared with control group (P < 0.05). Some neurons in ganglion cell layer (GCL) and nerve fibers in the innermost layer (fibrous layer) of the retina were also strongly positive. Fourteen days after ocular hypertension, the positive reaction products decreased compared with those in 7-day group, but still higher than that in normal control (P < 0.05). At the twenty-first day after ocular hypertension, the GAP-43 level in the retina recovered to the level of normal control. CONCLUSION: During chronic ocular hypertension, damage of retina and optic nerve triggers a transient upregulation of GAP-43 expression. LA: Chinese
Dr. X.-G. Yang, Department of Ophthalmology, The Fourth Hospital of Xi'an City, Shaanxi Provincial Ophthalmic Center, Xi'an 710004 Shaanxi Province, China. Yangxinguang.62@hotmail.com
Classification:
5.3 Other (Part of: 5 Experimental glaucoma; animal models)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
3.6 Cellular biology (Part of: 3 Laboratory methods)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)