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Abstract #22384 Published in IGR 10-4
Retinal arteriolar and capillary vascular reactivity in response to isoxic hypercapnia
Venkataraman ST; Hudson C; Fisher JA; Rodrigues L; Mardimae A; Flanagan JGExperimental Eye Research 2008; 87: 535-542
See also comment(s) by Alon Harris •
The aim of the study was to compare the magnitude of vascular reactivity of the retinal arterioles in terms of percentage change to that of the retinal capillaries using a novel, standardized methodology to provoke isoxic hypercapnia. Ten healthy subjects (mean age 25 years, range 21-31) were recruited. Subjects attended a single visit comprising two study sessions separated by 30 min. Subjects were fitted with a sequential re-breathing circuit connected to a computer-controlled gas blender. Each session consisted of breathing at rest for 10 min (baseline), increase of PETCO2 (maximum partial pressure of CO2 during expiration) by 15% above baseline whilst maintaining isoxia for 20 min, and returning to baseline conditions for 10 min. Retinal hemodynamic measurements were performed using the Canon Laser Blood Flowmeter and the Heidelberg Retina Flowmeter in random order across sessions. Retinal arteriolar diameter, blood velocity and flow increased by 3.3%, 16.9% and 24.9% (p < 0.001), respectively, during isoxic hypercapnia. There was also an increase of capillary blood flow of 34.8%, 21.6%, 24.9% (p ≤ 0.006) at the optic nerve head neuroretinal rim, nasal macula and fovea, respectively. The coefficient of repeatability (COR) was 5% of the average PETCO2 both at baseline and during isoxic hypercapnia and was 10% and 7% of the average PETO2 (minimum partial pressure of oxygen at end exhalation), respectively. The overall magnitude of retinal capillary vascular reactivity was equivalent to the arteriolar vascular reactivity with respect to percentage change of flow. The magnitude of isoxic hypercapnia was repeatable.
Dr. S.T. Venkataraman, Glaucoma & Retina Research Group, Department of Ophthalmology and Vision Sciences, University of Toronto, Ontario M5T 2S8, Canada