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Abstract #13262 Published in IGR 8-1
Neuroprotective effects of Erigeron Breviscapus (vant) Hand-Mazz on NMDA-induced retinal neuron injury in the rats
Shi J-M; Jiang Y-Q; Liu X-YInternational Journal of Ophthalmology 2005; 5: 859-863
AIM: To investigate whether Erigeron Breviscapus (vant) Hand-Mazz (EBHM) EBHM has neuroprotective effect against N-methyl-D-aspartate (NMDA)-induced neuron death in retinal ganglion cell layer (RGCL). METHODS: 60 healthy SD rats were randomly divided into four groups. 6 animals were normal control group (group A). The others were divided as group B (EBHM group), group C (normal saline+NMDA group) and group D (EBHM + NMDA group). Each group had 18 rats. 10nmol NMDA was intravitreally injected to induce partial damage of the neurons in RGCL in the right eyes of Groups C and D. Same volume PBS was intravitreally injected into the left eyes as self-control. Groups B and D were pre-treated intraperitoneally with 6g/L EBHM solution at a dose of 150mg/kg body weight/day seven days before and after NMDA treatment. Group C were administrated intraperitoneally with 9g/L normal saline at the same time of EBHM injection. Rats were sacrificed at 4, 7,14d after NMDA treatment. Flat whole retinas were stained with 5g/L cresyl violet and neuron counting in RGCL from both eyes were observed. Each subgroup had 6 rats. RESULTS: There was no significant difference of neuron counting in RGCL between the right eye and the left eye in group A (P = 0.200). There was no significant difference between normal control group and EBHM group either in the right eyes or in the left eyes at 4, 7 and 14 d respectively after intravitreal injection of 10nmol NMDA in group C and group D. (P = 0.636, P = 0.193). Neuron counting of RGCL in group C and D was significantly decreased in the NMDA-treated eyes at 4, 7 and 14d after intravitreal injection (P < 0.001). There was no significant difference between self-control eyes group and normal control group(P > 0.05). However, neuron counting was significantly higher in the EBHM+NMDA group than normal saline +NMDA group at 14days after intravitreal injection (P = 0.044), but was lowered than normal control group (P < 0.05). CONCLUSION: EBHM has no effect on neuron counting of RGCL when administered alone in normal rats. The results indicates that EBHM plays a partial protective role in NMDA-induced neuron loss in RGCL in the rats.
Dr. J.-M. Shi, Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Changsha 410011 Hunan Province, China
Classification:
11.8 Neuroprotection (Part of: 11 Medical treatment)
5 Experimental glaucoma; animal models