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1 General aspects (0)   1.1 Epidemiology (4)   1.2 Population genetics (0)   1.3 Pathogenesis (9)   1.4 Quality of life (7)   1.5 Glaucomas as cause of blindness (7)   1.6 Prevention and screening (3) 2 Anatomical structures in glaucoma (0)   2.1 Conjunctiva (3)   2.2 Cornea (23)   2.3 Sclera (1)   2.4 Anterior chamber angle (7)   2.5 Meshwork (0)     2.5.1 Trabecular meshwork (18)     2.5.2 Schlemms canal (0)     2.5.3 Scleral outlet channels (1)   2.6 Aqueous humor dynamics (0)     2.6.1 Production (0)     2.6.2 Outflow (0)       2.6.2.1 Trabecular meshwork (4)       2.6.2.2 Uveoscleral (1)     2.6.3 Compostion (8)   2.7 Episcleral veins and venous pressure (0)   2.8 Iris (3)   2.9 Ciliary body (2)   2.10 Lens (2)   2.11 Vitreous body (2)   2.12 Choroid, peripapillary choroid, peripapillary atrophy (3)   2.13 Retina and retinal nerve fibre layer (43)   2.14 Optic disc (18)   2.15 Optic nerve (3)   2.16 Chiasma and retrochiasmal central nervous system (0)   2.17 Stem cells (0)   2.20 Other (1) 3 Laboratory methods (0)   3.1 Microscopy (1)   3.2 Electron microscopy (0)   3.3 Immunohistochemistry (7)   3.4 Molecular genetics (0)     3.4.1 Linkage studies (0)     3.4.2 Gene studies (20)   3.5 Molecular biology incl. 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(12) 15 Miscellaneous (17)

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Abstract #20563 Published in IGR 10-1

Disease-related quantitation of TGF-β3 in human aqueous humor

Yoneda K; Nakano M; Mori K; Kinoshita S; Tashiro K
Growth Factors 2007; 25: 160-167

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TGF-β3 has been implicated in the pathology of ocular diseases, but its concentration in human aqueous humor has never been assessed. In this study, we established an enzyme-linked immunosorbent assay (ELISA) for TGF-β3 and quantitated it in aqueous humor collected from patients with pseudoexfoliation syndrome (PE), primary open angle glaucoma, chronic angle closure glaucoma and cataract (as the control). To develop the TGF-β3 ELISA, we screened antibodies to identify the best combination, validated the assay for aqueous humor, and optimized the procedure for preparing activated TGF-β3. As a result, our ELISA was highly selective and reproducible. Using our ELISA, we discovered a significantly elevated concentration of TGF-β3 in PE eyes. We also developed new TGF-β1 and -β2 ELISAs, and were able, for the first time, to quantitate all the TGF-β isoforms in the aqueous humor from a single eye, to assess their proportional effects on the pathogenesis of ocular diseases.

Dr. K. Tashiro, Department of Genomic Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto, Japan

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Classification:

2.6.3 Compostion (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics)
3.7 Biochemistry (Part of: 3 Laboratory methods)

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