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Abstract #21166 Published in IGR 10-2
C677T polymorphism in the methylenetetrahydrofolate reductase gene is associated with primary closed-angle glaucoma
Michael S; Qamar R; Akhtar F; Khan WA; Ahmed AMolecular Vision 2008; 14: 661-665
PURPOSE: To determine whether or not there is an association of the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism with disease in cohorts of primary open-angle glaucoma (POAG) and primary closed-angle glaucoma (PCAG) from Pakistan. METHODS: This was a prospective study consisting of 150 patients (90 POAG and 60 PCAG) and 70 control subjects. Genomic DNA was extracted from leukocytes of the peripheral blood. MTHFR C677T polymorphism analysis was performed by the polymerase chain reaction-restriction fragment length polymorphism (RFLP) technique. RESULTS: The prevalence of the MTHFR C/T genotype was 22.2% in POAG, 13.3% in PACG, and 18.6% in controls whereas the MTHFR T/T genotype was present solely in the PACG group (6.9%). The difference regarding the T/ T genotype between PACG and controls was statistically significant (p < 0.01). CONCLUSIONS: The MTHFR C677T polymorphism was found to be associated with PCAG but not POAG in patients of Pakistani origin.
Dr. A. Ahmed, Department of Biosciences, Comsats Institute of Information Technology, Islamabad, Capital Territory 44000, Pakistan. ahmedasifa@gmail.com
Classification:
3.4.2 Gene studies (Part of: 3 Laboratory methods > 3.4 Molecular genetics)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
9.3.1 Acute primary angle closure glaucoma (pupillary block) (Part of: 9 Clinical forms of glaucomas > 9.3 Primary angle closure glaucomas)
