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Abstract #14242 Published in IGR 8-3
Long-term effect of nonpenetrating filtering surgery with chitosan membrane implantation in rabbits
Li J; Li X-L; Huang Y-F; Hu PInternational Journal of Ophthalmology 2006; 6: 584-586
AIM: To evaluate effects of chitosan membrane on non-penetrating filtrating surgery (NPFS) in rabbits. METHODS: Chitosan membrane was extracted from the shell of snow-crab. Twenty-two New Zealand albino rabbits (44 eyes) were divided randomly into 2 groups: 22 eyes were used in group A and B respectively. The NPFS was performed in both group A and B, and chitosan implant was used only in group A. Clinical observation was performed including wound healing, inflammatory reaction, filtering and IOP. Pathological examination were taken from two eyes in each group at 1, 4, 12, 28, 36, 40 and 48 weeks, respectively. RESULTS: IOP: no obvious side effects we re noticed in group A and B before surgery (P > 0.05), but there were obvious different effects at different time points in each group and between group A and B (P < 0.01). UBM examination showed that the operating space was noticed in group A and B after surgery. At 36 weeks, partial fracture and absorption of chitosan implant was noticed and the space was small in group B. Histology observation: there was no significant statistical difference in the numbers of neutrophil between group A and B after surgery (P > 0.05). The numbers of phoroblast and eosinophil was noticed in two groups (P < 0.01). The percentage of total collagen and type- I collagen and type-III collagen was significant statistical difference (P < 0.01). CONCLUSION: Chitosan may effectively decrease IOP of animals and is suitable material for NPFS implants. LA: Chinese
Dr. X.-L. Li, Department of Ophthalmology, PLA General Hospital, Beijing 100853, China
Classification:
12.8.3 Non-perforating (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
