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Abstract #10046 Published in IGR 6-1
Transcript expression of matrix metalloproteinases in the conjunctiva following glaucoma filtration surgery in rabbits
Liu CJ; Huang YL; Chiu AW; Ju JPOphthalmic Research 2004; 36: 114-119
PURPOSE: To determine the changes in the expression of transcripts of matrix metalloproteinases (MMPs) and their tissue inhibitor (TIMP) in the conjunctiva following glaucoma filtration surgery (GFS). METHODS: The reverse transcription-polymerase chain reaction method was performed on tissue specimens obtained from adult rabbits undergoing GFS at various postoperative time points. RESULTS: MMP 1 decreased more than seven-fold from its baseline level during the first two weeks following surgery. MMP 2 decreased on the first postoperative day, but increased on day 2 and remained elevated for two weeks. The postoperative level of MMP 14 did not change much from baseline, except that noted on day 9. The TIMP 1 expression showed an early biphasic pattern. MMP 3 and MMP 9 were not detected in all the specimens. CONCLUSIONS: The transcript expression of MMPs and TIMP 1 in the conjunctiva is altered following GFS, with each gene product demonstrating a unique temporal pattern.
Dr. C.J. Liu, Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan. jlliu@vghtpe.gov.tw
Classification:
2.1 Conjunctiva (Part of: 2 Anatomical structures in glaucoma)
