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Abstract #17478 Published in IGR 9-2
Protective effect of extract of ginkgo biloba leaves on retinal neuron injury in chronic high intraocular presure rats
Li H-L; Li H; Han H-YInternational Journal of Ophthalmology 2007; 7: 414-416
AIM: To investigate the protective effect of extract of ginkgo biloba (EGb) on retinal ganglion cell layer neuron injury induced high intraocular pressure (IOP) in rats. METHODS: Sixty healthy Sprague-Dawley (SD) rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/(kg(bullet)d); group B, EGb 100mg/(kg(bullet)d); group C, EGb 150mg/(kg(bullet)d); and group D EGb 200mg/ (kg(bullet)d). After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer (RGCL) from both eyes of each rat were performed to evaluate neuron situation. RESULTS: There was no significant difference between the bilateral eyes on neuron counting from RGCL of normal control group (P > 0.05). There was no significant difference between the normal control group and the EGb treating normal IOP group on neuron counting from RGCL of (P > 0.05). The difference was significant between the experimental eyes of every dose group and normal control group (P < 0.05). The difference was significant between the experimental eyes and self-controlled eyes in every dose group (P < 0.05). The difference was significant between the experimental eyes of every and dose group and physiological brine treating group (P < 0.05). The difference was significant between the experimental eyes on neuron counting from RGCL of every dose group (P < 0.05). CONCLUSION: EGb has no effect on neuron counting of RGCL when administered alone in normal rats. EGb could partly provide the protective effect on retinal ganglion cell layer neuron injury induced chronic high IOP in rats. The protective effect of EGb will be enhanced according to the increasing dose in a definite range. LA: Chinese
Dr. H.-L. Li, Department of Opthalmology, Hospital of Nanhua University, Hengyang 421001 Hunan Province, China. lihong5056@yahoo.com.cn
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
11.8 Neuroprotection (Part of: 11 Medical treatment)
