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Abstract #26878 Published in IGR 12-3
Focused Conference Group: P02 - Transmembrane transport: Perspectives for disease and drug discovery calcium signal passway-based on to research the neuroprotection of effective components of traditional chinese medicine
Zhang Y; Lai X; Zhang JBasic and Clinical Pharmacology and Toxicology 2010; 107: 687
Glaucoma is the main blinding disease which foremost pathological change is optic nerve injure. Erigeron breviscapus, Chuanxiong and Radix peucedani are the objects of our project.We screen out the active parts or components which have Ca(2+) antagonistic property by 45Ca transmembrane influx technique, which are Seopoletin, Caffeic acid, Scutellarin, 3-O- dicaffeoylquinate, Quercetin, Apigenin, Erigeside, Chuanxiongzine hydrochloride, imperatiorin, isoimperatorin, Praeruptorin B, etc. The results of retinal neurons cultivated in normal atmosphere in vitro indicate that the dicaffeoyl extract from Erigeron breviscapus and isoimperatorin can promote retinal neurons alive in vitro. Ferulic acid, Verapamil and Chuanxiongzine hydrochloride can inhibit the apoptosis of retina gangliocyte caused by high pressure in vitro. The total flavonoids of erigeron breviscapus, breviscapine,imperatiorin,Praeruptorin B and Nimodipine can promote retinal neurons alive in vitro as well as inhibit the apoptosis of retina gangliocyte caused by high pressure in vitro. Combined Ca(2+) antagonistic experiments, effects on animals and relative references, we have found the optical neuroprotection and Ca(2+) antagonistic effect of erigeron breviscapus,chuanxiong and radix peucedani are corrrelated obviously. Furthermore, we have determined the optical neuroprotectional components of erigeron breviscapus by HPLCDAD fingerprints and phytochemistrical methods. Conclusively, our project provides new ideas for exploring the physical basis of Chinese medicine for glaucoma prevention and treatment, to develop innovative drugs based on the machanism of the optical neuroprotection.
Y. Zhang. Chengdu University of Traditional Chinese Medicine, National Medical College, ChengduChina.
Classification:
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
11.8 Neuroprotection (Part of: 11 Medical treatment)
