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Abstract #27226 Published in IGR 12-4
Neuroprotection of Copolymer 1 in glaucoma rats with elevated intraocular pressure
Sun J-F; Wang LJournal of Shanghai Jiaotong University Medical Science 2009; 29: 1196-1199
Objective: To observe the neuroprotective effect of Copolymer 1(Cop 1) in rats with elevated intraocular pressure (IOP), and explore the clinical application of Cop 1 in the prevention of optic nerve atrophy induced by glaucoma. Methods: Thirty adult Wistar rats were rendered for elevation of IOP in both eyes by Shareef-Sharma method. At the third week of IOP elevation, a mixture (1:1) of Cop 1 (100 (mu)g) emulsified in complete Freund's adjuvant (CFA) was injected intracutaneously at two hind footpads in 15 rats (Cop 1 model group), and the other 15 rats were given the same dose of PBS emulsified in CFA (model control group). Retinal ganglial cell (RGC) retrograde labeling was performed with Fluorogold 6 d later, and retina was taken after 24 h to compared the density of RGC between two groups. Another normal rats were served as blank controls. Results: It was found that lymphocytes were accumulated on the histological slices of retina and optic nerve in Cop 1 model group. The rates of RGC loss of Cop 1 model group and model control group were 10.24% (plus or minus) 3.75% and 29.00% (plus or minus) 6.92%, respectively. The RGC density in Cop 1 model group was significantly lower than that in model control group (P < 0.05), while there was no significant difference in that between Cop 1 model group and blank control group (P > 0.05). Conclusion: Intracutaneous injection of Cop 1 can reduce the damage of RGC induced by elevated IOP. LA: Chinese
J.-F. Sun. Department of Ophthalmology, Ruijin Hospital, Shanghai Jiaotong University, Shanghai 200025, China. sunjingfen@sohn.com
Classification:
11.8 Neuroprotection (Part of: 11 Medical treatment)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)