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Abstract #6828 Published in IGR 4-1
Prostaglandin F2 alpha can modulate the growth and the differentiation of bovine corneal epithelial cells cultured in vitro
Conconi MT; Spinazzi R; Tommasini M; Limoli A; Parnigotto PPAnnals of Anatomy 2001; 183: 567-573
The effects of PGF2α on the growth, morphology, morphometry and keratinization pattern of bovine corneal epithelial cells cultured in vitro were studied. The cells were grown with a basal medium or, in the presence of keratocytes and/or their products, using a keratocyte-conditioning medium. Cell growth was evaluated by MTT assay. Daily treatments with exogenous PGF2α at concentrations equal to or lower than 10-6 M induced significant increases in cell proliferation when the epithelial cells were cultured on a keratocyte feeder-layer or with the conditioning medium. No variations were observed in cultures grown with the basal medium. 10-5 M PGF2α induced a decrease in cell growth under all culturing conditions. PGF2α did not affect cell morphology and modified only nuclear dimensions among the cells grown under different culturing conditions. No variations of any parameters were observed between cells cultured on feeder-layer, with conditioning or basal medium and the corresponding cultures supplemented with the autacoid. Moreover, PGF2α only induced the disappearance of 43 kDa keratin in cells grown on basal medium, while the keratin pattern of epithelial cells cultured on feeder-layer or with the conditioning medium was not modified by the autacoid. From these data, we can suppose that a cooperation could exist between PGF2α and fibroblasts and their products for the modulation of cell growth. Finally, it was observed that the autacoid had no effect on cell morphology and morphometry, except for nuclear dimensions, despite the presence of other prostaglandins, such as PGE2.
Dr. M.T. Conconi, Department of Pharmaceutical Sciences, University of Padua, via Marzolo 5, I-35131 Padova, Italy
Classification:
11.4 Prostaglandins (Part of: 11 Medical treatment)
