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Abstract #25222 Published in IGR 12-1
Green Tea Catechins and Their Oxidative Protection in the Rat Eye
Chu KO; Chan KP; Wang CC; Chu CY; Li WY; Choy KW; Rogers MS; Pang CPJournal of agricultural and food chemistry 2010; 58: 1523–1534
Catechins, active constituents of green tea, are well-known antioxidative natural products. It was proposed that green tea extract (GTE) consumption could benefit the eye, and the pharmacokinetics of catechins and oxidation status in rat eye were investigated after oral administration. Sprague−Dawley rats were fed GTE and sacrificed at different time intervals. Their eyes were dissected into cornea, lens, retina, choroid-sclera, vitreous humor, and aqueous humor for analysis of catechins and 8-epi-isoprostane by HPLC-ECD and GC-NCI-MS, respectively. Catechins were differentially distributed in eye tissues. Gallocatechin was present at the highest concentration in the retina, 22729.4 ± 4229.4 pmol/g, and epigallocatechin in aqueous humor at 602.9 ± 116.7 nM. The corresponding area-under-curves were 207,000 pmol × h/g and 2035.0 ± 531.7 nM × h, respectively. The time of maximum concentration of the catechins varied from 0.5 to 12.2 h. Significant reductions in 8-epi-isoprostane levels were found in the compartments except the choroid-sclera or plasma, indicating antioxidative activities of catechins in these tissues.
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong Eye Hospital, Kowloon, Hong Kong
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
11.8 Neuroprotection (Part of: 11 Medical treatment)
